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Cascade-Enhanced Lateral Flow Immunoassay for Sensitive Detection of Okadaic Acid in Seawater, Fish, and Seafood.
- Source :
-
Foods (Basel, Switzerland) [Foods] 2022 Jun 09; Vol. 11 (12). Date of Electronic Publication: 2022 Jun 09. - Publication Year :
- 2022
-
Abstract
- In this investigation, a new approach for developing a sensitive lateral flow immunoassay (LFIA) was proposed for the detection of the hazardous marine toxin okadaic acid (OA). It is based on the indirect format with anti-species antibodies labeled by gold nanoparticles (AuNPs) and cascade signal amplification. The latter is performed by first passing a mixture of anti-OA antibodies and a tested sample along the immunochromatographic test strip and then performing several cycles of the interaction of anti-species antibodies conjugated with AuNPs with free antibodies, which bind to anti-species antibodies but are not specific to the target analyte. As a result, branched aggregates are formed, due to which the colorimetric signal intensification occurs. The developed test system enabled the detection of OA with an instrumental detection limit of 30 pg/mL and a cutoff of 1 ng/mL, which exceeds these characteristics in the LFIA without amplification by 7 and 2 times, respectively. The OA recoveries from seawater, fish, and seafood varied from 76.9% to 126%. The test system may be required for point-of-care monitoring of samples for phycotoxin contamination; the developed principle of signal amplification can be used in cases where highly sensitive detection of trace amounts of a contaminant is required.
Details
- Language :
- English
- ISSN :
- 2304-8158
- Volume :
- 11
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Foods (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 35741890
- Full Text :
- https://doi.org/10.3390/foods11121691