Evaluation of a pneumonia multiplex PCR panel for detection of bacterial respiratory tract pathogens from serial specimens collected from hospitalized COVID-19 patients.

Authors :: Tellapragada C
Ydsten KA
Ternhag A
Giske CG
Source :: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2022 Jul; Vol. 41 (7), pp. 1093-1098. Date of Electronic Publication: 2022 Jun 21.
Publication Year :: 2022
Abstract: We investigated the concordance between the Unyvero Hospitalized Pneumonia (HPN) application and quantitative culture for detection of bacterial pathogens from serial lower respiratory tract (LRT) specimens collected from the same subject. Comparison of results from HPN application and culture was evaluated using 69 LRT samples from 27 subjects, using two evaluation approaches. False positive detections by the HPN application was 29% (20/69) in Evaluation I vs 10% (7/68) in Evaluation II. Additional pathogens detected by the HPN application could be confirmed in many instances by culture positivity for the same organism from previous or subsequent samples from the same subject.<br /> (© 2022. The Author(s).)

Subjects :: Bacteria genetics
Humans
Multiplex Polymerase Chain Reaction methods
Respiratory System
Sensitivity and Specificity
COVID-19 diagnosis
Pneumonia diagnosis
Respiratory Tract Infections diagnosis
Respiratory Tract Infections microbiology

Language :: English
ISSN :: 1435-4373
Volume :: 41
Issue :: 7
Database :: MEDLINE
Journal :: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Publication Type :: Academic Journal
Accession number :: 35727430
Full Text :: https://doi.org/10.1007/s10096-022-04466-9

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