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Freeform Cell-Laden Cryobioprinting for Shelf-Ready Tissue Fabrication and Storage.

Authors :
Ravanbakhsh H
Luo Z
Zhang X
Maharjan S
Mirkarimi HS
Tang G
Chávez-Madero C
Mongeau L
Zhang YS
Source :
Matter [Matter] 2022 Feb 02; Vol. 5 (2), pp. 573-593. Date of Electronic Publication: 2021 Dec 21.
Publication Year :
2022

Abstract

One significant drawback of existing bioprinted tissues is their lack of shelf-availability caused by complications in both fabrication and storage. Here, we report a cryobioprinting strategy for simultaneously fabricating and storing cell-laden volumetric tissue constructs through seamlessly combining extrusion bioprinting and cryopreservation. The cryobioprinting performance was investigated by designing, fabricating, and storing cell-laden constructs made of our optimized cryoprotective gelatin-based bioinks using a freezing plate with precisely controllable temperature. The in situ freezing process further promoted the printability of cell-laden hydrogel bioinks to achieve freeform structures otherwise inconvenient with direct extrusion bioprinting. The effects of bioink composition on printability and cell viability were evaluated. The functionality of the method was finally investigated using cell differentiation and chick ex ovo assays. The results confirmed the feasibility and efficacy of cryobioprinting as a single-step method for concurrent tissue biofabrication and storage.<br />Competing Interests: Declaration of Interests The authors declare no competing interests.

Details

Language :
English
ISSN :
2590-2385
Volume :
5
Issue :
2
Database :
MEDLINE
Journal :
Matter
Publication Type :
Academic Journal
Accession number :
35695821
Full Text :
https://doi.org/10.1016/j.matt.2021.11.020