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Bio-Synthesis of Aspergillus terreus Mediated Gold Nanoparticle: Antimicrobial, Antioxidant, Antifungal and In Vitro Cytotoxicity Studies.
- Source :
-
Materials (Basel, Switzerland) [Materials (Basel)] 2022 May 29; Vol. 15 (11). Date of Electronic Publication: 2022 May 29. - Publication Year :
- 2022
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Abstract
- Gold nanoparticles (GNP) were bio-fabricated utilizing the methanolic extract of the endophytic isolate Aspergillus terreus . The biosynthesised gold nanoparticles (GNP023) were characterised using UV-visible spectroscopy (UV-Vis); transmission electron microscopy (TEM), Fourier-transform nfrared spectroscopy (FTIR) and X-ray diffraction (XRD) studies. The bio-fabricated GNP023 displayed a sharp SPR peak at 536 nm, were spherically shaped, and had an average size between 10-16 nm. The EDX profile confirmed the presence of gold (Au), and XRD analysis confirmed the crystalline nature of GNP023. The antimicrobial activity of GNP023 was investigated against several food-borne and phytopathogens, using in vitro antibacterial and antifungal assays. The maximum zone of inhibition was observed for S. aureus and V. cholera at 400 μg /mL, whereas inhibition in radial mycelial growth was observed against Fusarium oxysporum and Rhizoctonia solani at 52.5% and 65.46%, respectively, when challenged with GNP023 (200 μg/mL). Moreover, the gold nanoparticles displayed significant antioxidant activity against the ABTS radical, with an IC <subscript>50</subscript> of 38.61 µg/mL, and were non-toxic when tested against human kidney embryonic 293 (HEK293) cells. Thus, the current work supports the application of myco-synthesised gold nanoparticles as a versatile antimicrobial candidate against food-borne pathogens.
Details
- Language :
- English
- ISSN :
- 1996-1944
- Volume :
- 15
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Materials (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 35683175
- Full Text :
- https://doi.org/10.3390/ma15113877