Fabricate, advancement, molecular docking and DNA reactivity of preferred divalent metal(II) complexes attributing ( E )- N '-((6-hydroxybenzo[ d ]oxazol-5-yl) methylene)isonicotinohydrazide.

A series of metal(II) complexes ( M =Co (II), Ni(II) and Cu(II)) supported by Schiff base ligand ( L =( E )- N '-((6-hydroxybenzo[ d ]oxazol-5-yl)methylene)isonicotinohydrazide) has been designed and developed from condensation of 6-hydroxybenzo[ d ]oxazole-5-carbaldehyde and isoniazid. The ligand (H ₂ L) and its metal(II) complexes were structurally characterized utilizing a variety of physicochemical and spectroscopic approaches. The study shows that Schiff bases (H ₂ L) act as monobasic tridentate ONO ligand and conform to octahedral geometry according to the general formula [M(HL) ₂ ]. Furthermore, the interaction of these complexes with CT-DNA was investigated at pH = 7.2, utilizing UV-visible absorption, and viscosity measurement. In order to determine the mechanism of binding of the metal(II) complexes to the B-DNA dodecamer, docking studies were conducted using an AutoDock Vina 1.2.0 tool. The photo induced cleavage reveals that the ligand ( H ₂ L ) and its complexes have UV-visible photo nuclease properties against pUC19 DNA by agarose gel electrophoresis technique. Studies showed that the complexes evaluated firmly bind to CT-DNA via intercalative mode and provides a distinctive pattern of DNA binding.