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The Tarantula Toxin ω-Avsp1a Specifically Inhibits Human Ca V 3.1 and Ca V 3.3 via the Extracellular S3-S4 Loop of the Domain 1 Voltage-Sensor.
- Source :
-
Biomedicines [Biomedicines] 2022 May 04; Vol. 10 (5). Date of Electronic Publication: 2022 May 04. - Publication Year :
- 2022
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Abstract
- Inhibition of T-type calcium channels (Ca <subscript>V</subscript> 3) prevents development of diseases related to cardiovascular and nerve systems. Further, knockout animal studies have revealed that some diseases are mediated by specific subtypes of Ca <subscript>V</subscript> 3. However, subtype-specific Ca <subscript>V</subscript> 3 inhibitors for therapeutic purposes or for studying the physiological roles of Ca <subscript>V</subscript> 3 subtypes are missing. To bridge this gap, we employed our spider venom library and uncovered that Avicularia spec. ("Amazonas Purple", Peru) tarantula venom inhibited specific T-type Ca <subscript>V</subscript> channel subtypes. By using chromatographic and mass-spectrometric techniques, we isolated and sequenced the active toxin ω-Avsp1a, a C-terminally amidated 36 residue peptide with a molecular weight of 4224.91 Da, which comprised the major peak in the venom. Both native (4.1 μM) and synthetic ω-Avsp1a (10 μM) inhibited 90% of Ca <subscript>V</subscript> 3.1 and Ca <subscript>V</subscript> 3.3, but only 25% of Ca <subscript>V</subscript> 3.2 currents. In order to investigate the toxin binding site, we generated a range of chimeric channels from the less sensitive Ca <subscript>V</subscript> 3.2 and more sensitive Ca <subscript>V</subscript> 3.3. Our results suggest that domain-1 of Ca <subscript>V</subscript> 3.3 is important for the inhibitory effect of ω-Avsp1a on T-type calcium channels. Further studies revealed that a leucine of T-type calcium channels is crucial for the inhibitory effect of ω-Avsp1a.
Details
- Language :
- English
- ISSN :
- 2227-9059
- Volume :
- 10
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Biomedicines
- Publication Type :
- Academic Journal
- Accession number :
- 35625803
- Full Text :
- https://doi.org/10.3390/biomedicines10051066