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Antioxidant and Wound Healing Potential of Vitis vinifera Seeds Supported by Phytochemical Characterization and Docking Studies.
- Source :
-
Antioxidants (Basel, Switzerland) [Antioxidants (Basel)] 2022 Apr 29; Vol. 11 (5). Date of Electronic Publication: 2022 Apr 29. - Publication Year :
- 2022
-
Abstract
- This study explored the in vivo wound healing potential of Vitis vinifera seed extract using an excision wound model with focus on wound healing molecular targets including TGFBR1, VEGF, TNF-α, and IL-1β. The wound healing results revealed that V. vinifera seed extract enhanced wound closure rates (p < 0.001), elevated TGF-β and VEGF levels, and significantly downregulated TNF-α and IL-1β levels in comparison to the Mebo®-treated group. The phenotypical results were supported by biochemical and histopathological findings. Phytochemical investigation yielded a total of 36 compounds including twenty-seven compounds (1−27) identified from seed oil using GC-MS analysis, along with nine isolated compounds. Among the isolated compounds, one new benzofuran dimer (28) along with eight known ones (29−36) were identified. The structure of new compound was elucidated utilizing 1D/2D NMR, with HRESIMS analyses. Moreover, molecular docking experiments were performed to elucidate the molecular targets (TNF-α, TGFBR1, and IL-1β) of the observed wound healing activity. Additionally, the in vitro antioxidant activity of V. vinifera seed extract along with two isolated compounds (ursolic acid 34, and β-sitosterol-3-O-glucopyranoside 36) were explored. Our study highlights the potential of V. vinifera seed extract in wound repair uncovering the most probable mechanisms of action using in silico analysis.
Details
- Language :
- English
- ISSN :
- 2076-3921
- Volume :
- 11
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Antioxidants (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 35624745
- Full Text :
- https://doi.org/10.3390/antiox11050881