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Hyperspectral imaging for quantitative assessment of hepatic steatosis in human liver allografts.
- Source :
-
Clinical transplantation [Clin Transplant] 2022 Aug; Vol. 36 (8), pp. e14736. Date of Electronic Publication: 2022 Jun 05. - Publication Year :
- 2022
-
Abstract
- Introduction: In liver transplantation (LT), steatosis is commonly judged to be a risk factor for graft dysfunction, and quantitative assessment of hepatic steatosis remains crucial. Liver biopsy as the gold standard for evaluation of hepatic steatosis has certain drawbacks, that is, invasiveness, and intra- and inter-observer variability. A non-invasive, quantitative modality could replace liver biopsy and eliminate these disadvantages, but has not yet been evaluated in human LT.<br />Methods: We performed a pilot study to evaluate the feasibility and accuracy of hyperspectral imaging (HSI) in the assessment of hepatic steatosis of human liver allografts for transplantation. Thirteen deceased donor liver allografts were included in the study. The degree of steatosis was assessed by means of conventional liver biopsy as well as HSI, performed at the end of back-table preparation, during normothermic machine perfusion (NMP), and after reperfusion in the recipient.<br />Results: Organ donors were 51 [30-83] years old, and 61.5% were male. Donor body mass index was 24.2 [16.5-38.0] kg/m <superscript>2</superscript> . The tissue lipid index (TLI) generated by HSI at the end of back-table preparation correlated significantly with the histopathologically assessed degree of overall hepatic steatosis (R2 = .9085, P < .0001); this was based on a correlation of TLI and microvesicular steatosis (R2 = .8120; P < .0001). There is also a linear relationship between the histopathologically assessed degree of overall steatosis and TLI during NMP (R2 = .5646; P = .0031) as well as TLI after reperfusion (R2 = .6562; P = .0008).<br />Conclusion: HSI may safely be applied for accurate assessment of hepatic steatosis in human liver grafts. Certainly, TLI needs further assessment and validation in larger sample sizes.<br /> (© 2022 The Authors. Clinical Transplantation published by John Wiley & Sons Ltd.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Allografts pathology
Biopsy
Female
Humans
Hyperspectral Imaging
Liver diagnostic imaging
Liver pathology
Living Donors
Male
Middle Aged
Pilot Projects
Fatty Liver diagnostic imaging
Fatty Liver etiology
Liver Transplantation adverse effects
Liver Transplantation methods
Subjects
Details
- Language :
- English
- ISSN :
- 1399-0012
- Volume :
- 36
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Clinical transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 35622345
- Full Text :
- https://doi.org/10.1111/ctr.14736