CuO Nanozymes as Multifunctional Signal Labels for Efficiently Quenching the Photocurrent of ZnO/Au/AgSbS 2 Hybrids and Initiating a Strong Fluorescent Signal in a Dual-Mode Microfluidic Sensing Platform.

A novel dual-mode microfluidic sensing platform based on CuO nanozymes as a photoelectrochemical (PEC)-fluorescent (FL) multifunctional signal label was developed for ultrasensitive neuron specific enolase (NSE) detection. Herein, ZnO/Au/AgSbS ₂ hybrids, possessing excellent PEC properties, were first exploited as a sensing matrix to provide a stable photocurrent. The controlled synthesis of photoactive ZnO nanoflowers (NFs) was successfully conducted using a microfluidic reactor in the scale of seconds. Furthermore, the photocurrent of ZnO NFs decorated by Au and AgSbS ₂ nanoparticles significantly improved, owing to the local surface plasma resonance effect of Au and matching band structure between ZnO and AgSbS ₂ . A strategy of catalytic oxidation ascorbic acid (AA) by CuO nanozymes was proposed to quench the PEC signals and initiate FL signals. CuO nanoparticles growing on conductive carbon spheres (CuO@CSs) as secondary antibodies' labels could efficiently catalyze the oxidation of AA to achieve a PEC "signal-off" state. Then, the produced dehydroascorbic acid reacting with o -phenylenediamine opportunely generated a strong FL signal. Importantly, wide linear ranges of 0.0001-150 ng/mL for the PEC technique and 0.001-150 ng/mL for the FL method with a low detection limit of 0.028 and 0.25 pg/mL, respectively, could guarantee the sensitive detection of NSE.