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Microfluidic bioanalytical system for biofilm formation indication.
- Source :
-
Talanta [Talanta] 2022 Sep 01; Vol. 247, pp. 123541. Date of Electronic Publication: 2022 May 13. - Publication Year :
- 2022
-
Abstract
- The formation of biofilms is a key factor that researchers must consider when they work with bacterial cultures. We describe a new microfluidic bioanalytical sensory system for indicating biofilm formation. The method is demonstrated with Pseudomonas bacteria as an example and is based on the real-time recording of cell-polarizability changes caused by an alternating electric field. Control experiments using phase-contrast microscopy and traditional microbiological plating were done that proved biofilms had formed. The physical picture was described of the sensor-signal changes during cell transition from planktonic to biofilm growth. This transition was indicated by the appearance of a peak-shaped signal at 500 kHz and by an increase in the recorded relaxation time. Phenomena of increase in the signal relaxation time from 2.4 s for planktonic to 25.4 s for biofilm cells. The proposed microfluidic sensor system for indicating biofilm formation holds much promise, because it ensures an analysis time of about 20-30 min. An added bonus is that for this system there is no need to grow bacterial biofilms in a sensor and the flow cell is reusable.<br /> (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Subjects :
- Bacteria
Biofilms
Microfluidics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 247
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 35613523
- Full Text :
- https://doi.org/10.1016/j.talanta.2022.123541