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Idebenone regulates sirt1/Nrf2/TNF-α pathway with inhibition of oxidative stress, inflammation, and apoptosis in testicular torsion/detorsion in juvenile rats.
- Source :
-
Human & experimental toxicology [Hum Exp Toxicol] 2022 Jan-Dec; Vol. 41, pp. 9603271221102515. - Publication Year :
- 2022
-
Abstract
- Testicular torsion is an emergency, mainly in newborn and adolescent males, resulting in testicular ischemia. The current study aimed to evaluate the effect of Idebenone (IDE) on testicular torsion/detorsion (T/D) in juvenile rats. Thirty-two rats were randomized into: (1) the sham group: rats received sham operations with no other interventions; (2) the IDE group: rats received idebenone (100 mg/kg, i. p) without T/D; (3) the T/D group: rats underwent torsion for 2 h and detorsion for 4 h; and (4) the IDE+ T/D group: rats received IDE 1 h before T/D. Testicular malondialdehyde (MDA), total nitrite/nitrate (NOx), total antioxidant capacity (TAC), tumor necrosis factor-α (TNF-α), caspase-3, sirtuin type 1 (Sirt1), serum interleukin-1β (IL-1β), total cholesterol, and testosterone were measured. Histological changes, nuclear factor (erythroid-derived 2)-like-2 factors (Nrf2), and proliferating cell nuclear antigen (PCNA) immuno-expressions were assessed. T/D displayed an increase in MDA, NOx, TNF-α, caspase-3 , IL-1β, and total cholesterol with a significant decrease in TAC, Sirt1, and testosterone and strong positive Nrf2 and negative PCNA immuno-expressions. IDE could improve all oxidative, inflammatory, and apoptotic indicators. Therefore, IDE significantly reduced testicular ischemia-reperfusion injury in the juvenile rat testicular T/D model by limiting oxidative stress, inflammation, and apoptosis via the Sirt1/Nrf2/TNF-α pathway.
- Subjects :
- Adolescent
Animals
Antioxidants metabolism
Antioxidants pharmacology
Antioxidants therapeutic use
Apoptosis
Caspase 3 metabolism
Cholesterol
Humans
Inflammation metabolism
Male
Malondialdehyde metabolism
NF-E2-Related Factor 2 metabolism
Oxidative Stress
Proliferating Cell Nuclear Antigen metabolism
Rats
Rats, Wistar
Sirtuin 1 metabolism
Testis
Testosterone metabolism
Tumor Necrosis Factor-alpha metabolism
Ubiquinone analogs & derivatives
Reperfusion Injury metabolism
Spermatic Cord Torsion drug therapy
Spermatic Cord Torsion metabolism
Spermatic Cord Torsion pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1477-0903
- Volume :
- 41
- Database :
- MEDLINE
- Journal :
- Human & experimental toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 35593271
- Full Text :
- https://doi.org/10.1177/09603271221102515