Back to Search Start Over

The Melatonin Treatment Improves the Ovarian Responses After Superstimulation in Thai-Holstein Crossbreeds Under Heat Stress Conditions.

The Melatonin Treatment Improves the Ovarian Responses After Superstimulation in Thai-Holstein Crossbreeds Under Heat Stress Conditions.

Authors :
Ratchamak R
Thananurak P
Boonkum W
Semaming Y
Chankitisakul V
Source :
Frontiers in veterinary science [Front Vet Sci] 2022 Apr 28; Vol. 9, pp. 888039. Date of Electronic Publication: 2022 Apr 28 (Print Publication: 2022).
Publication Year :
2022

Abstract

The effect of heat stress with melatonin treatment on the superovulatory responses and embryo characteristics in Thai-Holstein crossbreeds under heat stress conditions was examined. Six non-lactating cows (replication = 4; n = 24) were assigned to one of 2 treatments in double cross-over design. All cows were superstimulated with traditional treatment. Melatonin group ( n = 12): cows received intramuscularly injection 18 mg/50 kg. simultaneously with GnRH injection, while those in the control group ( n = 12) received none. Bloods samples were taken to determine lipid peroxidation (MDA) and the activity of the antioxidant enzymes (superoxide dismutase; SOD). The experiment was conducted from April to September, which determined severe heat stress (the mean temperature-humidity index above 77). The results revealed that numbers of large follicles and corpora lutea were higher in the melatonin group than in the control group ( p < 0.01). Numbers of recovered ova/embryos, fertilized ova, and transferable embryos were higher in the melatonin group ( p < 0.01); meanwhile, more degenerated embryos were found in the control group ( p < 0.01). Increased activity of the antioxidant enzymes SOD after melatonin administration decreased MDA levels ( p < 0.05). In summary, melatonin administration benefited the ovarian response and embryo quality in superstimulated Thai-Holstein crossbreed affected by heat stress.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2022 Ratchamak, Thananurak, Boonkum, Semaming and Chankitisakul.)

Details

Language :
English
ISSN :
2297-1769
Volume :
9
Database :
MEDLINE
Journal :
Frontiers in veterinary science
Publication Type :
Academic Journal
Accession number :
35573411
Full Text :
https://doi.org/10.3389/fvets.2022.888039