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Lactobacillus reuteri improves function of the intestinal barrier in rats with acute liver failure through Nrf-2/HO-1 pathway.

Authors :
Zhou Q
Wu F
Chen S
Cen P
Yang Q
Guan J
Cen L
Zhang T
Zhu H
Chen Z
Source :
Nutrition (Burbank, Los Angeles County, Calif.) [Nutrition] 2022 Jul-Aug; Vol. 99-100, pp. 111673. Date of Electronic Publication: 2022 Apr 01.
Publication Year :
2022

Abstract

Objectives: We aimed to explore whether Lactobacillus reuteri could have a positive role in reducing inflammation and bacterial translocation in rats with acute liver failure.<br />Methods: Lactobacillus reuteri were gavaged to Sprague-Dawley (SD) rats at a dose of 1 × 10 <superscript>9</superscript> CFU/mL once a day for 14 d. D-galactosamine was injected intraperitoneally to induce acute liver failure for 24 h on the 15th day. Liver function, liver and ileum histology, intestinal cytokines, intestinal tight junction proteins, lipopolysaccharide binding protein, apoptosis molecules, and nuclear factor erythroid-derived 2 (Nrf-2) / heme oxygenase (HO-1) molecules were assessed.<br />Results: The results showed that L. reuteri alleviated liver injury and intestinal inflammation induced by D-galactosamine. L. reuteri also improved the expression of intestinal tight junction proteins and maintained the integrity of the intestinal barrier by inhibiting apoptosis of intestinal epithelial cells. L reuteri induced an increase in Nrf-2 nuclear translocation and elevated induction of HO-1. L. reuteri treatment significantly enhanced the expression of phosphoinositide 3-kinase/protein kinase B (PI3 K/Akt), protein kinase C (PKC), and their phosphorylated forms but not mitogen-activated protein kinase. The nuclear factor kappa B (NF-κB) pathway was inhibited after L. reuteri treatment. Interleukin (IL)-17A produced by Th17 cells and γδT17 cells may not contribute to an improved function of the intestinal barrier in L. reuteri-treated SD rats.<br />Conclusions: Overall, our study indicated that L. reuteri-induced expression of intestinal tight junction proteins is mediated by the PI3 K/Akt-Nrf-2/HO-1-NF-κB and PKC-Nrf-2/HO-1-NF-κB pathways, which leads to inhibition of the apoptosis of intestinal epithelial cells, thus maintaining the integrity of the damaged intestinal barrier.<br /> (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1873-1244
Volume :
99-100
Database :
MEDLINE
Journal :
Nutrition (Burbank, Los Angeles County, Calif.)
Publication Type :
Academic Journal
Accession number :
35567844
Full Text :
https://doi.org/10.1016/j.nut.2022.111673