A ratiometric fluorescent probe for detection of γ-glutamyl transpeptidase in blood serum and living cells.

Authors :: Wang S
Liu W
Zheng X
Ren H
Wu J
Li F
Wang P
Source :: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2022 Oct 05; Vol. 278, pp. 121325. Date of Electronic Publication: 2022 May 05.
Publication Year :: 2022
Abstract: γ-Glutamyl transpeptidase (GGT) is one of the biomarker of cancer, hepatitis, and numerous other diseases. The accurate analysis of GGT is useful for the early diagnosis of these diseases. In this work, Probe 1, a ratiometric fluorescent probe based on 2,3,5,6-tetrafluoroterephthalonitrile, was designed for GGT detection. The results indicated that Probe 1 can sensitively and selectively detect GGT in phosphate buffered solution and complex biological systems (e.g., blood serum). Furthermore, Probe 1 has been successfully applied for ratiometric imaging of GGT in cancer cells and normal cells.<br /> (Copyright © 2022 Elsevier B.V. All rights reserved.)

Subjects :: Hep G2 Cells
Humans
Serum chemistry
Fluorescent Dyes analysis
gamma-Glutamyltransferase analysis

Language :: English
ISSN :: 1873-3557
Volume :: 278
Database :: MEDLINE
Journal :: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Publication Type :: Academic Journal
Accession number :: 35567819
Full Text :: https://doi.org/10.1016/j.saa.2022.121325

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