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Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels.

Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels.

Authors :
Sevimli G
Smith MJ
Caglar TA
Bilir Ş
Secilmis M
Altun HY
Yigit EN
Yang F
Keeley TP
Malli R
Öztürk G
Mann GE
Eroglu E
Source :
Redox biology [Redox Biol] 2022 Jul; Vol. 53, pp. 102319. Date of Electronic Publication: 2022 Apr 30.
Publication Year :
2022

Abstract

Iron is an essential metal for cellular metabolism and signaling, but it has adverse effects in excess. The physiological consequences of iron deficiency are well established, yet the relationship between iron supplementation and pericellular oxygen levels in cultured cells and their downstream effects on metalloproteins has been less explored. This study exploits the metalloprotein geNOps in cultured HEK293T epithelial and EA.hy926 endothelial cells to test the iron-dependency in cells adapted to standard room air (18 kPa O <subscript>2</subscript> ) or physiological normoxia (5 kPa O <subscript>2</subscript> ). We show that cells in culture require iron supplementation to activate the metalloprotein geNOps and demonstrate for the first time that cells adapted to physiological normoxia require significantly lower iron compared to cells adapted to hyperoxia. This study establishes an essential role for recapitulating oxygen levels in vivo and uncovers a previously unrecognized requirement for ferrous iron supplementation under standard cell culture conditions to achieve geNOps functionality.<br /> (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
2213-2317
Volume :
53
Database :
MEDLINE
Journal :
Redox biology
Publication Type :
Academic Journal
Accession number :
35525027
Full Text :
https://doi.org/10.1016/j.redox.2022.102319