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The demonstration of rabies antigen in paraffin-embedded tissues using the peroxidase-antiperoxidase method: a comparative study.
- Source :
-
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire [Can J Vet Res] 1987 Jan; Vol. 51 (1), pp. 117-20. - Publication Year :
- 1987
-
Abstract
- Mice experimentally infected with challenge virus standard rabies virus as well as skunks and foxes experimentally infected with street rabies virus were used to demonstrate rabies viral antigen in paraffin-embedded tissue by the peroxidase-antiperoxidase method. Tissues fixed with different fixatives (10% formalin, Bouin's, acetone, ethanol) for various times and fresh frozen tissues were stained by the fluorescent antibody and the peroxidase-antiperoxidase method. Formalin- and Bouin's-fixed tissues were tested with and without use of digestive enzyme (pepsin). The results demonstrated that a procedure using formalin-fixed paraffin-embedded tissue treated with pepsin and stained by peroxidase-antiperoxidase was the best method for both preservation of morphological details and demonstration of antigen.
Details
- Language :
- English
- ISSN :
- 0830-9000
- Volume :
- 51
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
- Publication Type :
- Academic Journal
- Accession number :
- 3552169