Synthesis and characterisation of α-carboxynitrobenzyl photocaged l-aspartates for applications in time-resolved structural biology.

Authors :: Zaitsev-Doyle JJ
Puchert A
Pfeifer Y
Yan H
Yorke BA
Müller-Werkmeister HM
Uetrecht C
Rehbein J
Huse N
Pearson AR
Sans M
Source :: RSC advances [RSC Adv] 2019 Mar 15; Vol. 9 (15), pp. 8695-8699. Date of Electronic Publication: 2019 Mar 15 (Print Publication: 2019).
Publication Year :: 2019
Abstract: We report a new synthetic route to a series of α-carboxynitrobenzyl photocaged l-aspartates for application in time-resolved structural biology. The resulting compounds were characterised in terms of UV/Vis absorption properties, aqueous solubility and stability, and photocleavage rates ( τ = μs to ms) and quantum yields ( φ = 0.05 to 0.14).<br />Competing Interests: The authors declare no conflicts of interest.<br /> (This journal is © The Royal Society of Chemistry.)

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