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A magnet-actuated microfluidic array chip for high-throughput pretreatment and amplification and detection of multiple pathogens.
- Source :
-
The Analyst [Analyst] 2022 May 30; Vol. 147 (11), pp. 2433-2441. Date of Electronic Publication: 2022 May 30. - Publication Year :
- 2022
-
Abstract
- The outbreak of global infectious diseases has posed a significant threat to public health, requiring the rapid and accurate diagnosis of pathogens promptly for the society to implement immediate control measures to prevent widespread pandemics. In this work, a magnet-actuated microfluidic array chip (MMAC) is developed with integrated sample processing and nucleic acid amplification for the rapid detection of multiple pathogens by loop-mediated isothermal amplification. In comparison to previous works, where fluid control was dependent on external equipment or finger-based manual pressing, the fluid control of the MMAC is realized by magnetically actuating a ferric oxide (Fe <subscript>3</subscript> O <subscript>4</subscript> ) doped polydimethylsiloxane (PDMS) layer that separates the sample from the LAMP reagent in a high-throughput manner, which not only reduces the complexity of fluid control but also enhances the repeatability of detection by eliminating variations in operation by different users. Examination with a testing sample containing Salmonella typhimurium and Escherichia coli showed high specificity for pathogen detection without cross-contamination. The lowest detection concentration was 5.2 copies per μL for Salmonella typhimurium with a detection time of 60 min. The proposed method demonstrated the simultaneous detection of multiple pathogens, which is potentially helpful in applications of immediate diagnosis.
Details
- Language :
- English
- ISSN :
- 1364-5528
- Volume :
- 147
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 35510697
- Full Text :
- https://doi.org/10.1039/d2an00430e