GFI1 regulates chromatin state essential in human endothelial-to-haematopoietic transition.

Authors :: Kang B
Zhang T
Huang K
Wang T
Li Y
Mai Y
Li J
Dang S
Zhang Z
Huang W
Wang J
Gao M
Wang Y
Pan G
Source :: Cell proliferation [Cell Prolif] 2022 May; Vol. 55 (5), pp. e13244. Date of Electronic Publication: 2022 May 03.
Publication Year :: 2022
Abstract: Objectives: During embryonic haematopoiesis, haematopoietic stem/progenitor cells (HSPCs) develop from hemogenic endothelial cells (HECs) though endothelial to haematopoietic transition (EHT). However, little is known about how EHT is regulated in human. Here, we report that GFI1 plays an essential role in enabling normal EHT during haematopoietic differentiation of human embryonic stem cells (hESCs).<br />Results: GFI1 deletion in hESCs leads to a complete EHT defect due to a closed chromatin state of hematopoietic genes in HECs. Mechanically, directly regulates important signaling pathways essential for the EHT such as PI3K signaling.etc.<br />Conclutions: Together, our findings reveal an essential role of GFI1 mediated epigenetic mechanism underlying human EHT during hematopoiesis.<br /> (© 2022 The Authors. Cell Proliferation published by John Wiley & Sons Ltd.)

Subjects :: Cell Differentiation physiology
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
Hematopoiesis physiology
Humans
Phosphatidylinositol 3-Kinases metabolism
Transcription Factors genetics
Transcription Factors metabolism
Chromatin metabolism
Hemangioblasts metabolism

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