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Detection of DNA from Toxoplasma gondii oocysts in water for reuse.
- Source :
-
Parasitology research [Parasitol Res] 2022 Jul; Vol. 121 (7), pp. 2199-2203. Date of Electronic Publication: 2022 Apr 29. - Publication Year :
- 2022
-
Abstract
- The absence of a standardized method for detecting oocysts in water samples makes it difficult to characterize them, including in water for reuse. This study aimed to detect Toxoplasma gondii oocysts using two extraction methods. Using method 1693/2014 USEPA, 30 L of water for reuse from two wastewater treatment plants (WWTPs) in the city of São Paulo, Brazil, was concentrated, totaling 20 samples. The supernatant generated from the immunomagnetic separation (IMS) step was collected for detection of T. gondii oocysts. For DNA extraction, two techniques were used: the commercial kit DNeasy PowerSoil Kit® optimized with the enzyme Zymolyase® and with freeze-thaw steps. DNA quantification was performed with the target sequence of gene B1. From 16 samples submitted to enzymatic extraction, four were positive. In freeze-thaw extraction, no DNA was detected. DNA extraction was the essential step for oocyst detection given the resistant nature of their wall.<br /> (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Subjects :
- Animals
Brazil
DNA, Protozoan genetics
Oocysts
Water
Toxoplasma genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1955
- Volume :
- 121
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Parasitology research
- Publication Type :
- Academic Journal
- Accession number :
- 35486184
- Full Text :
- https://doi.org/10.1007/s00436-022-07528-1