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Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain.
- Source :
-
STAR protocols [STAR Protoc] 2022 Apr 15; Vol. 3 (2), pp. 101329. Date of Electronic Publication: 2022 Apr 15 (Print Publication: 2022). - Publication Year :
- 2022
-
Abstract
- Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).<br />Competing Interests: The authors declare no competing interests.<br /> (© 2022 The Author(s).)
Details
- Language :
- English
- ISSN :
- 2666-1667
- Volume :
- 3
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- STAR protocols
- Publication Type :
- Academic Journal
- Accession number :
- 35479117
- Full Text :
- https://doi.org/10.1016/j.xpro.2022.101329