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Analysis of memory B cells identifies conserved neutralizing epitopes on the N-terminal domain of variant SARS-Cov-2 spike proteins.
- Source :
-
Immunity [Immunity] 2022 Jun 14; Vol. 55 (6), pp. 998-1012.e8. Date of Electronic Publication: 2022 Apr 07. - Publication Year :
- 2022
-
Abstract
- SARS-CoV-2 infection or vaccination produces neutralizing antibody responses that contribute to better clinical outcomes. The receptor-binding domain (RBD) and the N-terminal domain (NTD) of the spike trimer (S) constitute the two major neutralizing targets for antibodies. Here, we use NTD-specific probes to capture anti-NTD memory B cells in a longitudinal cohort of infected individuals, some of whom were vaccinated. We found 6 complementation groups of neutralizing antibodies. 58% targeted epitopes outside the NTD supersite, 58% neutralized either Gamma or Omicron, and 14% were broad neutralizers that also neutralized Omicron. Structural characterization revealed that broadly active antibodies targeted three epitopes outside the NTD supersite including a class that recognized both the NTD and SD2 domain. Rapid recruitment of memory B cells producing these antibodies into the plasma cell compartment upon re-infection likely contributes to the relatively benign course of subsequent infections with SARS-CoV-2 variants, including Omicron.<br />Competing Interests: Declaration of interests The Rockefeller University has filed a provisional patent application in connection with this work on which M.C.N. and Z.W. are inventors (US patent 17/575,246).<br /> (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1097-4180
- Volume :
- 55
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Immunity
- Publication Type :
- Academic Journal
- Accession number :
- 35447092
- Full Text :
- https://doi.org/10.1016/j.immuni.2022.04.003