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Poly-L-lysine/hyaluronan nanocarriers as a novel nanosystem for gene delivery.
- Source :
-
Journal of microscopy [J Microsc] 2022 Jul; Vol. 287 (1), pp. 32-44. Date of Electronic Publication: 2022 Apr 29. - Publication Year :
- 2022
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Abstract
- The present research comes up with a novel DNA-loaded poly-L-lysine (PLL)/hyaluronan (HA) nanocarrier (DNA-loaded PLL/HA NCs) for gene delivery applications, as a promising candidate for gene delivery into diverse cells. A straightforward approach was employed to prepare such a nanosystem through masking DNA-loaded PLL molecules by HA. Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission-scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) were used to analyse the interaction of the molecules as well as the physicochemical properties of the NCs. The NCs showed a negative charge of -24 ± 3 mV, with an average size of 138 ± 6 nm, in an ellipsoid-shape with smooth surfaces. The DNA loading efficiency (LE) measured by DNA absorbance was around 95 %. The MTT assay showed that the developed NCs are non-toxic to the cells. Furthermore, the uptake of the DNA-loaded PLL/HA NCs by the human embryonic kidney (HEK)-293T cells was evaluated by a flow cytometry method, and demonstrated high potential cellular uptake over 90% for transferring the gene to HEK-293T cells at the optimised conditions. Therefore, the DNA-loaded PLL/HA NCs are the potent strategy for developing nanosystems for gene delivery applications.<br /> (© 2022 Royal Microscopical Society.)
Details
- Language :
- English
- ISSN :
- 1365-2818
- Volume :
- 287
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of microscopy
- Publication Type :
- Academic Journal
- Accession number :
- 35443072
- Full Text :
- https://doi.org/10.1111/jmi.13107