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Photosensitizer-loaded hydrogels for photodynamic inactivation of multirestistant bacteria in wounds.
- Source :
-
RSC advances [RSC Adv] 2021 Feb 17; Vol. 11 (13), pp. 7600-7609. Date of Electronic Publication: 2021 Feb 17 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Photodynamic treatment is a promising tool for the therapy of multidrug-resistant bacteria. In this study, we highlight photosensitizer-loaded hydrogels as an application system for infected wounds. The poly(ethylene glycol) diacrylate-based and electron beam-polymerized hydrogels were mechanically stable and transparent. They were loaded with two photoactive, porphyrin-based drugs - tetrakis(1 methylpyridinium-4-yl)porphyrin p -toluenesulfonate (TMPyP) and tetrahydroporphyrin - p toluenesulfonate (THPTS). The hydrogels released a sufficient amount of the photosensitizers (up to 300 μmol l <superscript>-1</superscript> ), relevant for efficiency. The antimicrobial effectivity of loaded hydrogels was investigated in a tissue-like system as well as in a liquid system against a multiresistant Escherichia coli . In both systems, light induced eradication was possible. In contrast, hydrogels alone showed only minor antimicrobial activity. Furthermore, the loaded hydrogels were successfully tested against seven multidrug-resistant bacterial strains, namely Enterococcus faecium , Staphylococcus aureus , Klebsiella pneumonia , Acinetobacter baumannii , Pseudomonas aeruginosa , Escherichia coli and Achromobacter xylosoxidans . The eradication of these pathogens, except A. xylosoxidans , was successfully demonstrated. In general, TMPyP-loaded hydrogels were more effective than THPTS-loaded ones. Nevertheless, both photosensitizers displayed effectivity against all investigated bacteria strains. Taken together, our data demonstrate that photosensitizer-loaded hydrogels are a promising new tool to improve the treatment of wounds infected with problematic bacterial pathogens.<br />Competing Interests: There are no conflicts to declare.<br /> (This journal is © The Royal Society of Chemistry.)
Details
- Language :
- English
- ISSN :
- 2046-2069
- Volume :
- 11
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- RSC advances
- Publication Type :
- Academic Journal
- Accession number :
- 35423227
- Full Text :
- https://doi.org/10.1039/d0ra09786a