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Quantitative Jones matrix imaging using vectorial Fourier ptychography.

Authors :
Dai X
Xu S
Yang X
Zhou KC
Glass C
Konda PC
Horstmeyer R
Source :
Biomedical optics express [Biomed Opt Express] 2022 Feb 14; Vol. 13 (3), pp. 1457-1470. Date of Electronic Publication: 2022 Feb 14 (Print Publication: 2022).
Publication Year :
2022

Abstract

This paper presents a microscopic imaging technique that uses variable-angle illumination to recover the complex polarimetric properties of a specimen at high resolution and over a large field-of-view. The approach extends Fourier ptychography, which is a synthetic aperture-based imaging approach to improve resolution with phaseless measurements, to additionally account for the vectorial nature of light. After images are acquired using a standard microscope outfitted with an LED illumination array and two polarizers, our vectorial Fourier ptychography (vFP) algorithm solves for the complex 2x2 Jones matrix of the anisotropic specimen of interest at each resolved spatial location. We introduce a new sequential Gauss-Newton-based solver that additionally jointly estimates and removes polarization-dependent imaging system aberrations. We demonstrate effective vFP performance by generating large-area (29 mm <superscript>2</superscript> ), high-resolution (1.24 μ m full-pitch) reconstructions of sample absorption, phase, orientation, diattenuation, and retardance for a variety of calibration samples and biological specimens.<br />Competing Interests: RH: Ramona Optics Inc. (F,I,P,S)<br /> (© 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.)

Details

Language :
English
ISSN :
2156-7085
Volume :
13
Issue :
3
Database :
MEDLINE
Journal :
Biomedical optics express
Publication Type :
Academic Journal
Accession number :
35414998
Full Text :
https://doi.org/10.1364/BOE.448804