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Real-time IR700 Fluorescence Imaging During Near-infrared Photoimmunotherapy Using a Clinically-approved Camera for Indocyanine Green.
- Source :
-
Cancer diagnosis & prognosis [Cancer Diagn Progn] 2021 May 03; Vol. 1 (2), pp. 29-34. Date of Electronic Publication: 2021 May 03 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Background/aim: Near-infrared photoimmuno-therapy (NIR-PIT) is a newly approved cancer phototherapy. We aim to confirm whether a clinically approved camera for indocyanine green (ICG) could monitor IR700 fluorescence in real time during exposure to therapeutic NIR light.<br />Materials and Methods: An NIR camera, LIGHTVISION, designed to image ICG fluorescence, was used. A431-GFP/luc tumor-bearing mice were exposed to therapeutic NIR light and real-time fluorescence imaging (RT-FI) was obtained and measured with LIGHTVISION. Bioluminescence imaging (BLI) was performed to confirm cell death.<br />Results: RT-FI during NIR-PIT revealed an initial rapid loss of fluorescence, followed by a plateau which occurred at a light dose of approximately 30 J/cm <superscript>2</superscript> . Correlation between BLI and IR700 fluorescence loss showed that loss of fluorescence was associated with increased cell death.<br />Conclusion: The efficacy of NIR-PIT could be monitored non-invasively and in real-time using weak fluorescence at wavelengths much longer than the peak fluorescence of IR700. This technique can achieve precise light dosimetry that allows us to decide on the optimal exposure.<br />Competing Interests: The Authors have no conflicts of interest to disclose.<br /> (Copyright 2021, International Institute of Anticancer Research.)
Details
- Language :
- English
- ISSN :
- 2732-7787
- Volume :
- 1
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cancer diagnosis & prognosis
- Publication Type :
- Academic Journal
- Accession number :
- 35403125
- Full Text :
- https://doi.org/10.21873/cdp.10005