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Epigenetic quantification of immunosenescent CD8 + TEMRA cells in human blood.
- Source :
-
Aging cell [Aging Cell] 2022 May; Vol. 21 (5), pp. e13607. Date of Electronic Publication: 2022 Apr 09. - Publication Year :
- 2022
-
Abstract
- Age-related changes in human T-cell populations are important contributors to immunosenescence. In particular, terminally differentiated CD8 <superscript>+</superscript> effector memory CD45RA <superscript>+</superscript> TEMRA cells and their subsets have characteristics of cellular senescence, accumulate in older individuals, and are increased in age-related chronic inflammatory diseases. In a detailed T-cell profiling among individuals over 65 years of age, we found a high interindividual variation among CD8 <superscript>+</superscript> TEMRA populations. CD8 <superscript>+</superscript> TEMRA proportions correlated positively with cytomegalovirus (CMV) antibody levels, however, not with the chronological age. In the analysis of over 90 inflammation proteins, we identified plasma TRANCE/RANKL levels to associate with several differentiated T-cell populations, including CD8 <superscript>+</superscript> TEMRA and its CD28 <superscript>-</superscript> subsets. Given the strong potential of CD8 <superscript>+</superscript> TEMRA cells as a biomarker for immunosenescence, we used deep-amplicon bisulfite sequencing to match their frequencies in flow cytometry with CpG site methylation levels and developed a computational model to predict CD8 <superscript>+</superscript> TEMRA cell proportions from whole blood genomic DNA. Our findings confirm the association of CD8 <superscript>+</superscript> TEMRA and its subsets with CMV infection and provide a novel tool for their high throughput epigenetic quantification as a biomarker of immunosenescence.<br /> (© 2022 The Authors. Aging Cell published by Anatomical Society and John Wiley & Sons Ltd.)
Details
- Language :
- English
- ISSN :
- 1474-9726
- Volume :
- 21
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Aging cell
- Publication Type :
- Academic Journal
- Accession number :
- 35397197
- Full Text :
- https://doi.org/10.1111/acel.13607