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The redox state of the plastoquinone (PQ) pool is connected to thylakoid lipid saturation in a marine diatom.
- Source :
-
Photosynthesis research [Photosynth Res] 2022 Aug; Vol. 153 (1-2), pp. 71-82. Date of Electronic Publication: 2022 Apr 07. - Publication Year :
- 2022
-
Abstract
- The redox state of the plastoquinone (PQ) pool is a known sensor for retrograde signaling. In this paper, we asked, "does the redox state of the PQ pool modulate the saturation state of thylakoid lipids?" Data from fatty acid composition and mRNA transcript abundance analyses suggest a strong connection between these two aspects in a model marine diatom. Fatty acid profiles of Phaeodactylum tricornutum exhibited specific changes when the redox state of the PQ pool was modulated by light and two chemical inhibitors [3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) or 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB)]. Data from liquid chromatography with tandem mass spectrometry (LC-MS/MS) indicated a ca. 7-20% decrease in the saturation state of all four conserved thylakoid lipids in response to an oxidized PQ pool. The redox signals generated from an oxidized PQ pool in plastids also increased the mRNA transcript abundance of nuclear-encoded C16 fatty acid desaturases (FADs), with peak upregulation on a timescale of 6 to 12 h. The connection between the redox state of the PQ pool and thylakoid lipid saturation suggests a heretofore unrecognized retrograde signaling pathway that couples photosynthetic electron transport and the physical state of thylakoid membrane lipids.<br /> (© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
- Subjects :
- Benzoquinones
Chromatography, Liquid
Dibromothymoquinone metabolism
Diuron pharmacology
Electron Transport
Fatty Acid Desaturases analysis
Fatty Acid Desaturases metabolism
Fatty Acids analysis
Light
Lipids
Oxidation-Reduction
RNA, Messenger metabolism
Tandem Mass Spectrometry
Thylakoids metabolism
Diatoms metabolism
Plastoquinone metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-5079
- Volume :
- 153
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Photosynthesis research
- Publication Type :
- Academic Journal
- Accession number :
- 35389175
- Full Text :
- https://doi.org/10.1007/s11120-022-00914-x