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Low concentrations of interleukin-1 stimulate and high concentrations inhibit insulin release from isolated rat islets of Langerhans.
- Source :
-
Acta endocrinologica [Acta Endocrinol (Copenh)] 1986 Dec; Vol. 113 (4), pp. 551-8. - Publication Year :
- 1986
-
Abstract
- Isolated rat islets were incubated either with crude, affinity-purified or recombinant human interleukin-1 for 1 to 6 days. A significant (20-60%) increase of insulin release was observed at low concentrations of all three interleukin-1-containing preparations. In contrast, higher concentrations dose-dependently inhibited the insulin release. The increased insulin secretion occurred at concentrations below those necessary to augment the mitogen response to phytohaemagglutinin of murine thymocytes in vitro. These doses (0.05-0.5 U/ml) correspond to 0.2-2 ng of recombinant interleukin-1 per ml, equal to approximately 0.01-0.1 pmol/ml. In doses of 0.6-1.8 U/ml affinity-purified interleukin-1 significantly increased the islet insulin content per ng of DNA, indicating a stimulation of insulin-biosynthesis. The data support the concept that low concentrations of interleukin-1 may play a role in priming the physiological secretion of insulin.
Details
- Language :
- English
- ISSN :
- 0001-5598
- Volume :
- 113
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Acta endocrinologica
- Publication Type :
- Academic Journal
- Accession number :
- 3538741
- Full Text :
- https://doi.org/10.1530/acta.0.1130551