Distribution and Magnitude of Regional Volumetric Lung Strain and Its Modification by PEEP in Healthy Anesthetized and Mechanically Ventilated Dogs.

The present study describes the magnitude and spatial distribution of lung strain in healthy anesthetized, mechanically ventilated dogs with and without positive end-expiratory pressure (PEEP). Total lung strain (LS _TOTAL ) has a dynamic (LS _DYNAMIC ) and a static (LS _STATIC ) component. Due to lung heterogeneity, global lung strain may not accurately represent regional total tissue lung strain (TS _TOTAL ), which may also be described by a regional dynamic (TS _DYNAMIC ) and static (TS _STATIC ) component. Six healthy anesthetized beagles (12.4 ± 1.4 kg body weight) were placed in dorsal recumbency and ventilated with a tidal volume of 15 ml/kg, respiratory rate of 15 bpm, and zero end-expiratory pressure (ZEEP). Respiratory system mechanics and full thoracic end-expiratory and end-inspiratory CT scan images were obtained at ZEEP. Thereafter, a PEEP of 5 cmH ₂ O was set and respiratory system mechanics measurements and end-expiratory and end-inspiratory images were repeated. Computed lung volumes from CT scans were used to evaluate the global LS _TOTAL , LS _DYNAMIC , and LS _STATIC during PEEP. During ZEEP, LS _STATIC was assumed zero; therefore, LS _TOTAL was the same as LS _DYNAMIC . Image segmentation was applied to CT images to obtain maps of regional TS _TOTAL , TS _DYNAMIC , and TS _STATIC during PEEP, and TS _DYNAMIC during ZEEP. Compliance increased ( p = 0.013) and driving pressure decreased ( p = 0.043) during PEEP. PEEP increased the end-expiratory lung volume ( p < 0.001) and significantly reduced global LS _DYNAMIC (33.4 ± 6.4% during ZEEP, 24.0 ± 4.6% during PEEP, p = 0.032). LS _STATIC by PEEP was larger than the reduction in LS _DYNAMIC ; therefore, LS _TOTAL at PEEP was larger than LS _DYNAMIC at ZEEP ( p = 0.005). There was marked topographic heterogeneity of regional strains. PEEP induced a significant reduction in TS _DYNAMIC in all lung regions ( p < 0.05). Similar to global findings, PEEP-induced TS _STATIC was larger than the reduction in TS _DYNAMIC ; therefore, PEEP-induced TS _TOTAL was larger than TS _DYNAMIC at ZEEP. In conclusion, PEEP reduced both global and regional estimates of dynamic strain, but induced a large static strain. Given that lung injury has been mostly associated with tidal deformation, limiting dynamic strain may be an important clinical target in healthy and diseased lungs, but this requires further study.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Araos, Cruces, Martin-Flores, Donati, Gleed, Boullhesen-Williams, Perez, Staffieri, Retamal, Vidal Melo and Hurtado.)