Effects of heavy metals on growth and biofilm-producing abilities of Salmonella enterica isolated from Tunisia.

This study aims to test the toxicity of some metallic elements on Salmonella enterica strains and their power to grow and to develop a biofilm to overcome this environmental stress. From 50 selected strains of Salmonella, 70% belong to the Kentucky serotypes that is the most frequent one, followed by the other serotypes such as Amsterdam 6%, anatum 4%, derby 4% Enteritidis 4%, Zanzibar 4%, typhyrimium 2%, gallinaruim 2%, inbondaka 2% and Newport 2%. All the strains have presented the invA invasion gene involved in the virulence and Salmonella infection. Genotypic BOX-PCR analysis of these strains showed 18 profiles, with a discrimination index of 0.93. The Salmonella growth has mainly revealed that the variation of the rates of different metallic elements showed a significant influence on the Salmonella growth. The qualitative, quantitative study and biofilm tubes showed that 40% of the strains have a strong capacity to form biofilm, and the wild-type phenotypes (RDAR; rigid film; Strong), This phenotype varies according to the nature and the concentration of the metal (0.1 mM-1 mM) considered. In the presence of copper, zinc, cobalt, and chromium, the Salmonella strains showed a potent capacity to form a biofilm with a slight variation in the wild-type phenotype. However, when chromium rates increased, Salmonella loses the RDAR morphotype. Addition of mercury and cadmium in the growth medium reduced the production of Salmonella biofilm by around 14 and 15%, respectively, if compared with the control free of metals.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)