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RNA Sequencing of Lens Capsular Epithelium Implicates Novel Pathways in Pseudoexfoliation Syndrome.

Authors :
Mullany S
Marshall H
Zhou T
Thomson D
Schmidt JM
Qassim A
Knight LSW
Hollitt G
Berry EC
Nguyen T
To MS
Dimasi D
Kuot A
Dubowsky J
Fogarty R
Sun M
Chehade L
Kuruvilla S
Supramaniam D
Breen J
Sharma S
Landers J
Lake S
Mills RA
Hassall MM
Chan WO
Klebe S
Souzeau E
Siggs OM
Craig JE
Source :
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2022 Mar 02; Vol. 63 (3), pp. 26.
Publication Year :
2022

Abstract

Purpose: Pseudoexfoliation syndrome (PEX) is a common systemic disease that results in severe and often irreversible vision loss. Despite considerable research effort, PEX remains incompletely understood. This study sought to perform the first RNAseq study in elucidate the pathophysiology of PEX, and contribute a publicly available transcriptomic data resource for future research.<br />Methods: Human ocular lens capsular epithelium samples were collected from 25 patients with PEX and 39 non-PEX controls undergoing cataract surgery. RNA extracted from these specimens was subjected to polyadenylated (mRNA) selection and deep bulk RNA sequencing. Differential expression analysis investigated protein-coding gene transcripts. Exploratory analyses used pathway analysis tools, and curated class- and disease-specific gene sets.<br />Results: Differential expression analysis demonstrated that 2882 genes were differentially expressed according to PEX status. Genes associated with viral gene expression pathways were among the most upregulated, alongside genes encoding ribosomal and mitochondrial respiratory transport chain proteins. Cell adhesion protein transcripts including type 4 collagen subunits were downregulated.<br />Conclusions: This comparative transcriptomic dataset highlights novel and previously recognized pathogenic pathways in PEX and provides the first comprehensive transcriptomic resource, adding an additional layer to build further understanding of PEX pathophysiology.

Details

Language :
English
ISSN :
1552-5783
Volume :
63
Issue :
3
Database :
MEDLINE
Journal :
Investigative ophthalmology & visual science
Publication Type :
Academic Journal
Accession number :
35348588
Full Text :
https://doi.org/10.1167/iovs.63.3.26