Cellulosimicrobium protaetiae sp. nov., isolated from the gut of the larva of Protaetia brevitarsis seulensis .

A Gram-stain-positive, non-spore-forming, yellow-pigmented, non-motile, non-flagellated, facultative anaerobic and rod-shaped bacterial strain, designated BI34 ^T , was isolated from the gut of the larva of Protaetia brevitarsis seulensis . Strain BI34 ^T grew at 15-40 °C (optimum, 37 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-7 % (w/v) NaCl (optimum, 2 %). Based on the results of 16S rRNA gene sequence analysis, strain BI34 ^T belonged to the phylum Actinobacteria and was closely related to Cellulosimicrobium funkei NBRC 104118 ^T (99.3 %), Cellulosimicrobium cellulans NBRC 15516 ^T (99.1 %), Cellulosimicrobium composti BIT-GX5 ^T (99.0 %), Cellulosimicrobium fucosivorans SE3 ^T (99.0 %), Cellulosimicrobium marinum NBRC 110994 ^T (98.4 %) and Cellulosimicrobium terreum DS-61 ^T (97.0 %). The genome to genome relatedness of the average nucleotide identity (ANI) and the digital DNA-DNA hybridization (dDDH) values calculated by the Genome-to-Genome Distance Calculator between strain BI34 ^T and its related species mentioned above were lower than the threshold of 95 and 70 % for speciation, respectively. The predominant menaquinone of strain BI34 ^T contained MK-9(H ₄ ), and the major fatty acids were anteiso-C _15 : 0 , C _16 : 0 and anteiso-C _17 : 0 . Strain BI34 ^T had diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. The whole-cell sugars were galactose, glucose and ribose, and the cell-wall peptidoglycan contained lysine, alanine, aspartic acid and glutamic acid. The DNA G+C content of strain BI34 ^T was 73.8 mol%. The difference in physiological and biochemical characteristics and the below-threshold values of genome-to-genome relatedness indicate that strain BI34 ^T represents a novel species in the genus Cellulosimicrobium , for which the name Cellulosimicrobium protaetiae sp. nov. is proposed. The type strain is BI34 ^T (=KCTC 49302 ^T =NBRC 114073 ^T ).