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Aptapaper─An Aptamer-Functionalized Glass Fiber Paper Platform for Rapid Upconcentration and Detection of Small Molecules.
- Source :
-
Analytical chemistry [Anal Chem] 2022 Apr 12; Vol. 94 (14), pp. 5651-5657. Date of Electronic Publication: 2022 Mar 28. - Publication Year :
- 2022
-
Abstract
- We tested a paper-based platform ("Aptapaper") for the upconcentration and analysis of small molecules from complex matrices for two well-characterized aptamers, quinine and serotonin binding aptamers (QBA and SBA, respectively). After incubating the aptapaper under conditions that ensure correct aptamer folding, the aptapaper was used to upconcentrate target analytes from complex matrices. Aptapaper was rinsed, dried, and the target analyte was detected immediately or up to 4 days later by paper spray ionization coupled to high-resolution mass spectrometry (PS-MS). The minimum concentrations detectable were 81 pg/mL and 1.8 ng/mL for quinine and serotonin, respectively, from 100 mM AmAc or water. Complementary characterization of the QBA aptapaper system was performed using an orthogonal fluorescence microscopy method. Random adsorption was analyte-specific and observed for quinine, but not serotonin. This aptapaper approach is a semiquantitative (10-20% RSD) platform for upconcentration of small metabolites by mass spectrometry.
- Subjects :
- Glass
Mass Spectrometry methods
Quinine
Serotonin
Aptamers, Nucleotide chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 94
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 35343676
- Full Text :
- https://doi.org/10.1021/acs.analchem.2c00035