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Development of a microtiter plate-based analysis method of nitric oxide dioxygenase activity.
- Source :
-
The Journal of general and applied microbiology [J Gen Appl Microbiol] 2022 Jun 20; Vol. 68 (1), pp. 38-41. Date of Electronic Publication: 2022 Mar 23. - Publication Year :
- 2022
-
Abstract
- Nitric oxide (NO) functions in cell protection or cell death, depending on its concentration. Therefore, regulation of the intracellular concentrations of NO by its degradation systems is important for cellular functions. One of the NO degrading enzymes, flavohemoglobin (FHb), which has NO dioxygenase (NOD) activity, is a promising target for antibiotics, based on the finding that FHb-deficient pathogens exhibited reduced host toxicity. Here, we developed a high-throughput method to measure the NOD activity. Our newly developed method could contribute to the screening of potential antibiotics with NOD inhibitory activity.
- Subjects :
- Anti-Bacterial Agents pharmacology
Nitric Oxide metabolism
Oxygenases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1349-8037
- Volume :
- 68
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of general and applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 35321969
- Full Text :
- https://doi.org/10.2323/jgam.2021.07.001