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Metastatic human hepatoblastoma cells exhibit enhanced tumorigenicity, invasiveness and a stem cell-like phenotype.

Authors :
Marayati R
Julson JR
Bownes LV
Quinn CH
Hutchins SC
Williams AP
Markert HR
Beierle AM
Stewart JE
Hjelmeland AB
Mroczek-Musulman E
Beierle EA
Source :
Journal of pediatric surgery [J Pediatr Surg] 2022 Jun; Vol. 57 (6), pp. 1018-1025. Date of Electronic Publication: 2022 Feb 14.
Publication Year :
2022

Abstract

Background/purpose: Metastatic hepatoblastoma continues to pose a significant treatment challenge, primarily because the precise mechanisms involved in metastasis are not fully understood, making cell lines and preclinical models that depict the progression of disease and metastasis-related biology paramount. We aimed to generate and characterize a metastatic hepatoblastoma cell line to create a model for investigation of the molecular mechanisms associated with metastasis.<br />Materials/methods: Using a murine model of serial tail vein injections of the human hepatoblastoma HuH6 cell line, non-invasive bioluminescence imaging, and dissociation of metastatic pulmonary lesions, we successfully established and characterized the metastatic human hepatoblastoma cell line, HLM_3.<br />Results: The HLM_3 cells exhibited enhanced tumorigenicity and invasiveness, both in vitro and in vivo compared to the parent HuH6 cell line. Moreover, HLM_3 metastatic hepatoblastoma cells exhibited a stem cell-like phenotype and were more resistant to the standard chemotherapeutic cisplatin.<br />Conclusion: This newly described metastatic hepatoblastoma cell line offers a novel tool to study mechanisms of tumor metastasis and evaluate new therapeutic strategies for metastatic hepatoblastoma.<br /> (Copyright © 2022 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1531-5037
Volume :
57
Issue :
6
Database :
MEDLINE
Journal :
Journal of pediatric surgery
Publication Type :
Academic Journal
Accession number :
35300860
Full Text :
https://doi.org/10.1016/j.jpedsurg.2022.01.063