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Secondary structure as primary determinant of the efficiency of ribosomal binding sites in Escherichia coli.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1986 Jul 11; Vol. 14 (13), pp. 5481-97. - Publication Year :
- 1986
-
Abstract
- Using a previously described vector (pKL203) we fused several heterologous ribosomal binding sites (RBSs) to the lacZ gene of E. coli and then studied the variation in expression of the fusions. The RBSs originated from bacteriophage Q beta and MS2 genes and the E. coli genes for elongation factor EF-Tu A and B and ribosomal protein L11 (rplK). The synthesis of the lacZ fusion proteins was measured by an immuno precipitation method and found to vary at least 100-fold. Lac-specific mRNA synthesis follows the variation in protein production. It appears that there is a correlation between the efficiency of an RBS to function in the expression of the fused gene and the lack of secondary structure, involving the Shine and Dalgarno nucleotides (SDnts) and/or the initiation codon. This efficiency is context dependent. The sequence of the SD nts and the length and sequence of the spacer region up to the initiation codon alone are not able to explain our results. Deletion mutations, created in the phage Q beta replicase RBS, reveal a complex pattern of control of expression, probably involving the use of a "false" initiation site.
- Subjects :
- Bacterial Proteins genetics
Base Sequence
Binding Sites
Cloning, Molecular
Gene Expression Regulation
Genes, Bacterial
Genes, Viral
Lac Operon
Nucleic Acid Conformation
Structure-Activity Relationship
Viral Proteins genetics
Escherichia coli physiology
RNA, Messenger metabolism
Ribosomes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 14
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 3526283
- Full Text :
- https://doi.org/10.1093/nar/14.13.5481