Role of Ca 2+ , Calnexin and Calreticulin in Platelet from Adult Patients with Chronic Immune Thrombocytopenic Purpura.

Background: Adult chronic immune thrombocytopenia (chronic ITP) is a common autoimmune hemorrhagic disease characterized by decreased platelet production and increased platelet destruction, leading to thrombocytopenia. In this study, Ca ²⁺ , calnexin (CNX) and calreticulin (CRT) within platelets from adult patients with chronic ITP were investigated.
Methods: Platelets were isolated from blood specimen collected from 20 adult patients with chronic ITP and 20 healthy volunteers. Ca ²⁺ , CNX and CRT were determined by flow cytometry, and the results were analyzed with EXPO32 ADC software.
Results: Flow cytometry showed the expressions of Ca ²⁺ (74.19±19.40% vs 22.79±10.47%) was elevated ( P <0.05). However, CNX (15.10±7.32% vs 41.79±14.45%) and CRT (25.11±12.66% vs 38.58±12.02%) were decreased markedly in platelets from adult patients with chronic ITP ( P <0.05 compared with healthy volunteers).
Conclusion: Based on enhanced expression of Ca ²⁺ and attenuated expression of CNX and CRT in patients with chronic ITP, Ca ²⁺ concentration and its associated down-regulated proteins may be important regulatory signals in the pathogenesis of chronic ITP.
Competing Interests: Da-Ming Xu and Ze-Wen Zhang are co-first authors for this study. The authors do not have any conflicts of interest to report in this work.
(© 2022 Xu et al.)