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Investigating the Intercellular Communication Network of Immune Cell in Acute Respiratory Distress Syndrome with Sepsis.
- Source :
-
Computational and mathematical methods in medicine [Comput Math Methods Med] 2022 Feb 16; Vol. 2022, pp. 4586648. Date of Electronic Publication: 2022 Feb 16 (Print Publication: 2022). - Publication Year :
- 2022
-
Abstract
- Acute respiratory distress syndrome (ARDS) is recognized as a serious public health issue that results in respiratory failure and high mortality rates. The syndrome is characterized by immune cell aggregation, communication, activation, and alveolar epithelial damage. To elucidate the complex dynamic process of the immune system's response in ARDS, we construct the intercellular communication network of immune cells in ARDS based on a single-cell RNA sequencing dataset (including three sepsis-induced ARDS patients and four sepsis-only patients). The results show that macrophages relayed most of the intercellular signals (ligand-receptor pairs) in both groups. Many genes related to immune response (IFI44L, ISG, and HLA-DQB1) and biological functions (response to virus, negative regulation of viral life cycle, and response to interferon-beta) were detected via differentially expressed gene analysis of macrophages between the two groups. Deep analysis of the intercellular signals related to the macrophage found that sepsis-induced ARDS harbored distinctive intercellular signals related to chemokine-chemokine receptors (CCL3/4/5-CCR1), which mainly are involved in the disturbance of the STAT family transcription factors (TFs), such as STAT2 and STAT3. These signals and downstream TFs might play key roles in macrophage M1/M2 polarization in the process of sepsis-induced ARDS. This study provides a comprehensive view of the intercellular communication landscape between sepsis and sepsis-induced ARDS and identifies key intercellular communications and TFs involved in sepsis-induced ARDS. We believe that our study provides valuable clues for understanding the immune response mechanisms of ARDS.<br />Competing Interests: The authors declare that they have no conflicts of interest.<br /> (Copyright © 2022 Pei Tao et al.)
- Subjects :
- Cell Communication genetics
Computational Biology
Databases, Nucleic Acid
Gene Expression
Gene Ontology
Humans
Macrophages immunology
RNA-Seq
Respiratory Distress Syndrome etiology
Respiratory Distress Syndrome genetics
Sepsis complications
Sepsis genetics
Single-Cell Analysis
Cell Communication immunology
Respiratory Distress Syndrome immunology
Sepsis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1748-6718
- Volume :
- 2022
- Database :
- MEDLINE
- Journal :
- Computational and mathematical methods in medicine
- Publication Type :
- Academic Journal
- Accession number :
- 35222683
- Full Text :
- https://doi.org/10.1155/2022/4586648