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Impact of Collagen Crosslinking on Dislocated Human Shoulder Capsules-Effect on Structural and Mechanical Properties.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2022 Feb 18; Vol. 23 (4). Date of Electronic Publication: 2022 Feb 18. - Publication Year :
- 2022
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Abstract
- Classical treatments of shoulder instability are associated with recurrence. To determine whether the modification of the capsule properties may be an alternative procedure, the effect of crosslinking treatment on the structure and mechanical properties of diseased human shoulder capsules was investigated. Joint capsules harvested from patients during shoulder surgery (n = 5) were treated or not with UV and/or riboflavin (0.1%, 1.0% and 2.5%). The structure and the mechanical properties of the capsules were determined by atomic force microscopy. The effect of treatments on cell death was investigated. Collagen fibrils were well-aligned and adjacent to each other with a D-periodicity of 66.9 ± 3.2 nm and a diameter of 71.8 ± 15.4 nm in control untreated capsules. No effect of treatments was observed on the organization of the collagen fibrils nor on their intrinsic characteristics, including D-periodicity or their mean diameter. The treatments also did not induce cell death. In contrast, UV + 2.5% riboflavin induced capsule stiffness, as revealed by the increased Young's modulus values ( p < 0.0001 for each patient). Our results showed that the crosslinking procedure changed the biomechanics of diseased capsules, while keeping their structural organisation unchanged at the single fibril level. The UV/riboflavin crosslinking procedure may be a promising way to preserve the functions of collagen-based tissues and tune their elasticity for clinically relevant treatments.
- Subjects :
- Biomechanical Phenomena drug effects
Cross-Linking Reagents chemistry
Elastic Modulus drug effects
Elasticity drug effects
Extracellular Matrix drug effects
Humans
Joint Instability
Microscopy, Atomic Force methods
Riboflavin chemistry
Riboflavin pharmacology
Ultraviolet Rays
Collagen chemistry
Collagen pharmacology
Cross-Linking Reagents pharmacology
Shoulder physiology
Shoulder Joint drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 23
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 35216412
- Full Text :
- https://doi.org/10.3390/ijms23042297