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Automated Grooming Detection of Mouse by Three-Dimensional Convolutional Neural Network.

Authors :
Sakamoto N
Kobayashi K
Yamamoto T
Masuko S
Yamamoto M
Murata T
Source :
Frontiers in behavioral neuroscience [Front Behav Neurosci] 2022 Feb 02; Vol. 16, pp. 797860. Date of Electronic Publication: 2022 Feb 02 (Print Publication: 2022).
Publication Year :
2022

Abstract

Grooming is a common behavior for animals to care for their fur, maintain hygiene, and regulate body temperature. Since various factors, including stressors and genetic mutations, affect grooming quantitatively and qualitatively, the assessment of grooming is important to understand the status of experimental animals. However, current grooming detection methods are time-consuming, laborious, and require specialized equipment. In addition, they generally cannot discriminate grooming microstructures such as face washing and body licking. In this study, we aimed to develop an automated grooming detection method that can distinguish facial grooming from body grooming by image analysis using artificial intelligence. Mouse behavior was recorded using a standard hand camera. We carefully observed videos and labeled each time point as facial grooming, body grooming, and not grooming. We constructed a three-dimensional convolutional neural network (3D-CNN) and trained it using the labeled images. Since the output of the trained 3D-CNN included unlikely short grooming bouts and interruptions, we set posterior filters to remove them. The performance of the trained 3D-CNN and filters was evaluated using a first-look dataset that was not used for training. The sensitivity of facial and body grooming detection reached 81.3% and 91.9%, respectively. The positive predictive rates of facial and body grooming detection were 83.5% and 88.5%, respectively. The number of grooming bouts predicted by our method was highly correlated with human observations (face: r = 0.93, body: r = 0.98). These results highlight that our method has sufficient ability to distinguish facial grooming and body grooming in mice.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2022 Sakamoto, Kobayashi, Yamamoto, Masuko, Yamamoto and Murata.)

Details

Language :
English
ISSN :
1662-5153
Volume :
16
Database :
MEDLINE
Journal :
Frontiers in behavioral neuroscience
Publication Type :
Academic Journal
Accession number :
35185488
Full Text :
https://doi.org/10.3389/fnbeh.2022.797860