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Mechanism of spindle pole organization and instability in human oocytes.
- Source :
-
Science (New York, N.Y.) [Science] 2022 Feb 11; Vol. 375 (6581), pp. eabj3944. Date of Electronic Publication: 2022 Feb 11. - Publication Year :
- 2022
-
Abstract
- Human oocytes are prone to assembling meiotic spindles with unstable poles, which can favor aneuploidy in human eggs. The underlying causes of spindle instability are unknown. We found that NUMA (nuclear mitotic apparatus protein)-mediated clustering of microtubule minus ends focused the spindle poles in human, bovine, and porcine oocytes and in mouse oocytes depleted of acentriolar microtubule-organizing centers (aMTOCs). However, unlike human oocytes, bovine, porcine, and aMTOC-free mouse oocytes have stable spindles. We identified the molecular motor KIFC1 (kinesin superfamily protein C1) as a spindle-stabilizing protein that is deficient in human oocytes. Depletion of KIFC1 recapitulated spindle instability in bovine and aMTOC-free mouse oocytes, and the introduction of exogenous KIFC1 rescued spindle instability in human oocytes. Thus, the deficiency of KIFC1 contributes to spindle instability in human oocytes.
- Subjects :
- 1-Alkyl-2-acetylglycerophosphocholine Esterase metabolism
Animals
Cattle
Dynactin Complex metabolism
Dyneins metabolism
Female
Humans
Kinesins genetics
Kinesins metabolism
Mice
Microtubule-Associated Proteins metabolism
Microtubule-Organizing Center physiology
Microtubule-Organizing Center ultrastructure
Microtubules metabolism
Recombinant Proteins metabolism
Spindle Apparatus ultrastructure
Spindle Poles ultrastructure
Swine
Cell Cycle Proteins metabolism
Kinesins deficiency
Oocytes physiology
Oocytes ultrastructure
Spindle Apparatus physiology
Spindle Poles physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 375
- Issue :
- 6581
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 35143306
- Full Text :
- https://doi.org/10.1126/science.abj3944