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Bioprocess development for bacterial cellulose biosynthesis by novel Lactiplantibacillus plantarum isolate along with characterization and antimicrobial assessment of fabricated membrane.
- Source :
-
Scientific reports [Sci Rep] 2022 Feb 09; Vol. 12 (1), pp. 2181. Date of Electronic Publication: 2022 Feb 09. - Publication Year :
- 2022
-
Abstract
- Bacterial cellulose (BC) is an ecofriendly biopolymer with diverse commercial applications. Its use is limited by the capacity of bacterial production strains and cost of the medium. Mining for novel organisms with well-optimized growth conditions will be important for the adoption of BC. In this study, a novel BC-producing strain was isolated from rotten fruit samples and identified as Lactiplantibacillus plantarum from 16S rRNA sequencing. Culture conditions were optimized for supporting maximal BC production using one variable at a time, Plackett-Burman design, and Box Behnken design approaches. Results indicated that a modified Yamanaka medium supported the highest BC yield (2.7 g/l), and that yeast extract, MgSO <subscript>4</subscript> , and pH were the most significant variables influencing BC production. After optimizing the levels of these variables through Box Behnken design, BC yield was increased to 4.51 g/l. The drug delivery capacity of the produced BC membrane was evaluated through fabrication with sodium alginate and gentamycin antibiotic at four different concentrations. All membranes (normal and fabricated) were characterized by scanning electron microscope, Fourier transform-infrared spectroscopy, X-ray diffraction, and mechanical properties. The antimicrobial activity of prepared composites was evaluated by using six human pathogens and revealed potent antibacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Streptococcus mutans, with no detected activity against Pseudomonas aeruginosa and Candida albicans.<br /> (© 2022. The Author(s).)
- Subjects :
- Alginates pharmacology
Anti-Bacterial Agents pharmacology
Bacteria drug effects
Cellulose chemistry
Cellulose isolation & purification
Culture Media
Gentamicins pharmacology
Lactobacillaceae isolation & purification
Lactobacillaceae metabolism
Microscopy, Electron, Scanning
Polysaccharides, Bacterial biosynthesis
Polysaccharides, Bacterial chemistry
Spectroscopy, Fourier Transform Infrared
Surface Properties
X-Ray Diffraction
Anti-Infective Agents pharmacology
Cell Culture Techniques methods
Cellulose biosynthesis
Lactobacillaceae chemistry
Lactobacillaceae genetics
Membranes chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 35140278
- Full Text :
- https://doi.org/10.1038/s41598-022-06117-7