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Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling†.
- Source :
-
Biology of reproduction [Biol Reprod] 2022 May 17; Vol. 106 (5), pp. 888-899. - Publication Year :
- 2022
-
Abstract
- Given recent reports of expression of postnatal mineral transport regulators at the maternal-conceptus interface during the peri-implantation period, this study tested the hypothesis that progesterone (P4) and interferon tau (IFNT) regulate phosphate, calcium, and vitamin D signaling in the ovine endometrium. Mature Rambouillet ewes (n = 24) were surgically fitted with intrauterine catheters on day 7 of the estrous cycle. Ewes received daily intramuscular injections of 50 mg of P4 in corn oil vehicle and 75 mg of progesterone receptor antagonist (RU486) in corn oil from days 8 to 15, and twice-daily intrauterine injections of either control proteins (CX) or IFNT (25 μg/uterine horn/day) from days 11 to 15 resulting in four treatment groups: P4 + CX; P4 + IFNT; RU486 + P4 + CX; and RU486 + P4 + IFNT. On day 16, ewes were hysterectomized. RU486 + P4 + CX treated ewes had lower concentrations of 25 (OH) D in plasma than P4 + CX treated ewes (P < 0.05). Endometria from ewes treated with IFNT had greater expression of FGF23 (P < 0.01), S100A9 (P < 0.05), and S100A12 (P = 0.05) mRNAs and lower expression of ADAM10 mRNA (P < 0.01) than of ewes treated with CX proteins. Expression of FGF23 mRNA was greater in endometria of ewes that received RU486 + P4 + IFNT than in ewes that received RU486 + P4 + CX (hormone × protein interaction, P < 0.05). The expression of S100G mRNA was greater in endometria of ewes that received P4 + IFNT compared to ewes that received RU486 + P4 + IFNT (P < 0.05; hormone × protein interaction, P < 0.01). These data implicate P4 and IFNT in the regulation of phosphate, calcium, and vitamin D signaling during the peri-implantation period of pregnancy and provide a platform for continued mechanistic investigations.<br /> (© The Author(s) 2022. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Subjects :
- Animals
Calcium metabolism
Corn Oil metabolism
Corn Oil pharmacology
Endometrium metabolism
Female
Mifepristone pharmacology
Phosphates metabolism
Phosphates pharmacology
Pregnancy
Pregnancy Proteins
Proteins metabolism
RNA, Messenger metabolism
Sheep
Sheep, Domestic
Vitamin D pharmacology
Interferon Type I metabolism
Progesterone metabolism
Progesterone pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1529-7268
- Volume :
- 106
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Biology of reproduction
- Publication Type :
- Academic Journal
- Accession number :
- 35134855
- Full Text :
- https://doi.org/10.1093/biolre/ioac027