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Metabolomics analysis of freeze-thaw tolerance enhancement mechanism of ε-poly-l-lysine on industrial yeast.
- Source :
-
Food chemistry [Food Chem] 2022 Jul 15; Vol. 382, pp. 132315. Date of Electronic Publication: 2022 Feb 02. - Publication Year :
- 2022
-
Abstract
- Antimicrobial polycationic peptide ε-poly-l-lysine (ε-PL) enhanced the freeze-thaw tolerance of industrial yeast; the enhancement mechanism of ε-PL on yeast was studied. Results showed that a ε-PL coating was observed in ε-PL-treated yeast. After 4 times of freeze-thaw, the cell viability, glycerol content, and CO <subscript>2</subscript> production of 0.6 mg/mL ε-PL-treated yeast were higher than those of untreated yeast, specifically, the cell viability of ε-PL-treated yeast was 87.6%, and that of untreated yeast was 68.5%. Metabolomic results showed that the enhancement mechanism of ε-PL on yeast was related to the promotion of cell membrane-related fatty acid synthesis pathways before freeze-thaw treatment, and the promotion of trehalose biosynthesis and glycerophospholipid metabolism pathways after freeze-thaw. Furthermore, ε-PL induced inhibition of the tricarboxylic acid cycle, resulting in a longer stationary phase at the beginning of the freeze-thaw and ultimately providing a higher level of freeze-thaw stress tolerance than untreated yeast.<br /> (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Subjects :
- Freezing
Metabolomics
Polylysine
Saccharomyces cerevisiae metabolism
Yeast, Dried
Subjects
Details
- Language :
- English
- ISSN :
- 1873-7072
- Volume :
- 382
- Database :
- MEDLINE
- Journal :
- Food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 35134720
- Full Text :
- https://doi.org/10.1016/j.foodchem.2022.132315