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Performance of the Abbott SARS-CoV-2 IgG serological assay in South African 2 patients.
- Source :
-
PloS one [PLoS One] 2022 Feb 04; Vol. 17 (2), pp. e0262442. Date of Electronic Publication: 2022 Feb 04 (Print Publication: 2022). - Publication Year :
- 2022
-
Abstract
- In late December 2019, pneumonia cases of unknown origin were reported in Wuhan, China. This virus was named SARS-CoV2 and the clinical syndrome was named coronavirus disease 19 (COVID-19). South Africa, despite strict and early lockdown has the highest infection rate in Africa. A key component of South Africa's response to SARSCoV2 was the rapid scale-up of diagnostic testing. The Abbott SARS-CoV2 assay detects IgG antibodies against the Nucleocapsid (N) protein of the SARS-CoV2 virus. This study undertook to validate and evaluate performance criteria of the Abbott assay and to establish whether this assay would show clinical utility in our population. Positive patients (n = 391) and negative controls (n = 139) were included. The Architect-i and Alinity-i systems were analyzers that were used to perform the SARS-CoV-2 IgG assay. In-house ELISA was incorporated into the study as a confirmatory serology test. A total of number of 530 participants was tested, 87% were symptomatic with infection and 13% were asymptomatic. When compared to RT-qPCR, the sensitivity of Architect and Alinity SARS-CoV2 assays was 69.5% and 64.8%, respectively. Specificity for Architect and Alinity assays was 95% and 90.3%, respectively. The Abbott assay was also compared to in house ELISA assay, with sensitivity for the Architect and Alinity assays of 94.7% and 92.5%, respectively. Specificity for Abbott Alinity assays was 91.7% higher than Abbott Architect 88.1%. Based on the current findings testing of IgG after 14 days is recommended in South Africa and supports other studies performed around the world.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Adult
Aged
Aged, 80 and over
Antibodies, Viral immunology
COVID-19 blood
COVID-19 epidemiology
COVID-19 virology
Enzyme-Linked Immunosorbent Assay
Female
Follow-Up Studies
Humans
Immunoglobulin G immunology
Male
Middle Aged
Prognosis
Retrospective Studies
South Africa epidemiology
Young Adult
Antibodies, Viral blood
COVID-19 diagnosis
COVID-19 Serological Testing methods
Immunoglobulin G blood
SARS-CoV-2 isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 17
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 35120133
- Full Text :
- https://doi.org/10.1371/journal.pone.0262442