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Comprehensive characterization and molecular insights into the salt tolerance of a Cu, Zn-superoxide dismutase from an Indian Mangrove, Avicennia marina.
- Source :
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Scientific reports [Sci Rep] 2022 Feb 02; Vol. 12 (1), pp. 1745. Date of Electronic Publication: 2022 Feb 02. - Publication Year :
- 2022
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Abstract
- Superoxide dismutases are important group of antioxidant metallozyme and play important role in ROS homeostasis in salinity stress. The present study reports the biochemical properties of a salt-tolerant Cu, Zn-superoxide from Avicennia marina (Am&#95;SOD). Am&#95;SOD was purified from the leaf and identified by mass-spectrometry. Recombinant Am&#95;SOD cDNA was bacterially expressed as a homodimeric protein. Enzyme kinetics revealed a high substrate affinity and specific activity of Am&#95;SOD as compared to many earlier reported SODs. An electronic transition in 360-400 nm spectra of Am&#95;SOD is indicative of Cu <superscript>2+</superscript> -binding. Am&#95;SOD activity was potentially inhibited by diethyldithiocarbamate and H <subscript>2</subscript> O <subscript>2</subscript> , a characteristic of Cu, Zn-SOD. Am&#95;SOD exhibited conformational and functional stability at high NaCl concentration as well in alkaline pH. Introgression of Am&#95;SOD in E. coli conferred tolerance to oxidative stress under highly saline condition. Am&#95;SOD was moderately thermostable and retained functional activity at ~ 60 °C. In-silico analyses revealed 5 solvent-accessible N-terminal residues of Am&#95;SOD that were less hydrophobic than those at similar positions of non-halophilic SODs. Substituting these 5 residues with non-halophilic counterparts resulted in > 50% reduction in salt-tolerance of Am&#95;SOD. This indicates a cumulative role of these residues in maintaining low surface hydrophobicity of Am&#95;SOD and consequently high salt tolerance. The molecular information on antioxidant activity and salt-tolerance of Am&#95;SOD may have potential application in biotechnology research. To our knowledge, this is the first report on salt-tolerant SOD from mangrove.<br /> (© 2022. The Author(s).)
- Subjects :
- Escherichia coli genetics
Escherichia coli metabolism
Mass Spectrometry
Organisms, Genetically Modified
Oxidative Stress physiology
Plant Leaves metabolism
Salt Stress physiology
Avicennia genetics
Avicennia metabolism
Salt Tolerance physiology
Superoxide Dismutase chemistry
Superoxide Dismutase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 35110640
- Full Text :
- https://doi.org/10.1038/s41598-022-05726-6