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Preliminary results using a kit to measure tamoxifen and metabolites concentrations in capillary blood samples from women with breast cancer.
- Source :
-
Scientific reports [Sci Rep] 2022 Jan 31; Vol. 12 (1), pp. 1643. Date of Electronic Publication: 2022 Jan 31. - Publication Year :
- 2022
-
Abstract
- The aim of the study was to compare 3 blood sampling methods, including capillary blood sampling, for determining Tamoxifen (TAM), Z-endoxifen (END), and 4-hydroxytamoxifen (4HT) concentrations. High performance liquid chromatography-mass spectrometry was used to quantify concentrations of TAM, END, and 4HT in plasma, venous blood, and capillary blood samples of 16 participants on TAM therapy for breast cancer. The rhelise kit was used for capillary sampling. Calibration curves using <superscript>13</superscript> C-labeled analogs of TAM, END, and 4HT as internal standards were used for quantifications. A capillary sampling kit was used successfully for all participants. Mean TAM concentrations did not differ significantly in the 3 types of samples. Mean END and 4HT concentrations did differ significantly between capillary and venous blood samples, possibly related to photodegradation in the internal standards prior to use or degradation products with chromatographic retention times similar to the metabolites. TAM, END, and 4HT concentrations were relatively stable when stored for 14 days at 8 °C and 20 °C. Therapeutic drug monitoring of TAM using an innovative kit and capillary blood sampling is feasible. Preliminary data from this study will aid in developing a multicenter, randomized clinical trial of personalized TAM dose monitoring and adjustments, with the goal of enhancing the quality-of-life and outcomes of patients with breast cancer.Clinical Trial Identification: EudraCT No 2017-000641-44.<br /> (© 2022. The Author(s).)
- Subjects :
- Adult
Aged
Aged, 80 and over
Breast Neoplasms drug therapy
Capillaries
Chromatography, High Pressure Liquid
Estrogen Antagonists therapeutic use
Feasibility Studies
Female
Humans
Mass Spectrometry
Middle Aged
Pilot Projects
Predictive Value of Tests
Reproducibility of Results
Sweden
Tamoxifen blood
Tamoxifen therapeutic use
Breast Neoplasms blood
Drug Monitoring instrumentation
Estrogen Antagonists blood
Reagent Kits, Diagnostic
Tamoxifen analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 35102224
- Full Text :
- https://doi.org/10.1038/s41598-022-05443-0