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Geminiviruses employ host DNA glycosylases to subvert DNA methylation-mediated defense.
- Source :
-
Nature communications [Nat Commun] 2022 Jan 31; Vol. 13 (1), pp. 575. Date of Electronic Publication: 2022 Jan 31. - Publication Year :
- 2022
-
Abstract
- DNA methylation is an epigenetic mechanism that plays important roles in gene regulation and transposon silencing. Active DNA demethylation has evolved to counterbalance DNA methylation at many endogenous loci. Here, we report that active DNA demethylation also targets viral DNAs, tomato yellow leaf curl China virus (TYLCCNV) and its satellite tomato yellow leaf curl China betasatellite (TYLCCNB), to promote their virulence. We demonstrate that the βC1 protein, encoded by TYLCCNB, interacts with a ROS1-like DNA glycosylase in Nicotiana benthamiana and with the DEMETER (DME) DNA glycosylase in Arabidopsis thaliana. The interaction between βC1 and DME facilitates the DNA glycosylase activity to decrease viral DNA methylation and promote viral virulence. These findings reveal that active DNA demethylation can be regulated by a viral protein to subvert DNA methylation-mediated defense.<br /> (© 2022. The Author(s).)
- Subjects :
- Arabidopsis virology
DNA, Viral metabolism
Plant Diseases virology
Plant Proteins metabolism
Protein Binding
Satellite Viruses pathogenicity
Nicotiana virology
Viral Proteins metabolism
Virulence
Begomovirus pathogenicity
DNA Glycosylases metabolism
DNA Methylation genetics
Host-Pathogen Interactions genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 35102164
- Full Text :
- https://doi.org/10.1038/s41467-022-28262-3