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Circular RNA 0000654 facilitates the growth of gastric cancer cells through absorbing microRNA-149-5p to up-regulate inhibin-beta A.
- Source :
-
Bioengineered [Bioengineered] 2022 Jan; Vol. 13 (1), pp. 469-480. - Publication Year :
- 2022
-
Abstract
- Circular (circ) RNAs are differentially expressed in gastric cancer (GC) and participate in the biological growth of tumor cells. Given that, investigations were performed to unravel the function of circ_0000654 in GC. GC tissue and normal tissue specimens were obtained, in which circ_0000654, microRNA (miR)-149-5p, and inhibin-beta A (INHBA) levels were examined. GC cell line (BGC-823) was transfected to alter circ_0000654 and miR-149-5p expression, thereby observing cell malignancy. Stably-transfected BGC-823 cells were injected into nude mice to observe tumor growth in vivo . The interaction circ_0000654, miR-149-5p, and INHBA was validated. circ_0000654 and INHBA were up-regulated but miR-149-5p was down-regulated in GC. circ_0000654 absorbed miR-149-5p to target INHBA. Silencing circ_0000654inhibited the progress of GC cell biology. Oppositely, restoring circ_0000654 enhanced the growth of GC cells. Inhibiting miR-149-5p rescued down-regulated circ_0000654-induced anti-tumor effect on GC. circ_0000654 silence or miR-149-5p overexpression limited the growth of GC tumors in vivo . Obviously, circ_0000654 facilitates the growth of GC cells through absorbing miR-149-5p to up-regulate INHBA.
- Subjects :
- Cell Line, Tumor
Humans
Inhibin-beta Subunits genetics
MicroRNAs genetics
Neoplasm Proteins genetics
RNA, Circular genetics
RNA, Neoplasm genetics
Stomach Neoplasms genetics
Inhibin-beta Subunits biosynthesis
MicroRNAs biosynthesis
Neoplasm Proteins biosynthesis
RNA, Circular biosynthesis
RNA, Neoplasm biosynthesis
Stomach Neoplasms metabolism
Up-Regulation
Subjects
Details
- Language :
- English
- ISSN :
- 2165-5987
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Bioengineered
- Publication Type :
- Academic Journal
- Accession number :
- 35100076
- Full Text :
- https://doi.org/10.1080/21655979.2021.2009414